Polyvalent and monoclonal antibodies identify major immunogenic proteins specific for human herpesvirus 7-infected cells and have weak cross-reactivity with human herpesvirus 6.
作者： L. Foa-Tomasi , E. Avitabile , L. Ke , G. Campadelli-Fiume
DOI: 10.1099/0022-1317-75-10-2719
关键词: Cross-reactivity 、 Glycoprotein 、 Virology 、 Virus 、 Antigenicity 、 Immune system 、 Molecular biology 、 Herpesviridae 、 Monoclonal antibody 、 Biology 、 Human herpesvirus 6
摘要: Hyperimmune rabbit and mouse sera raised to human herpesvirus 7 (HHV-7)-infected cells an immune serum identified 20 [35S]methionine-[35S]cysteinelabelled proteins specific for HHV-7-infected cord blood mononuclear cells, ranging in apparent M r from 136K 30K. The major had values of 121K, 100K, 87K, 85K, 60K, 51K, 46K, 42K, 40K 36K. also seven [3H]glycosamine-labelled glycoproteins, with 89K, 82K, 67K, 63K, 53K 41K. Four monoclonal antibodies (MAbs) were derived. Two reacted a family five antigenically related polypeptides (87K, 70K, 61K 57K M r), designated as the p85 complex. 121K 51K p121 p51, respectively. Human react high frequency complex lesser extent p121; hence these two appear be immunodominant both humans laboratory animals. hyperimmune some MAbs showed cross-reactivity HHV-6A(U1102)- 6B(Z29)-infected cells. implications respect response HHV-6 -7 infections prevalence analyses are discussed.
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