Protective role of the L-arginine-nitric oxide synthase pathway on preservation injury after rat liver transplantation.

摘要: Background A major problem complicating liver transplantation is the preservation injury that results from cold storage and subsequent ischemia/reperfusion after organ revascularization. The L-arginine-nitric oxide (NO) pathway has been recognized to play critical roles during infection, inflammation, injury, transplant rejection. Recent data indicates NO synthesis beneficial effects in several models of injury. purpose this study examine role L-arginine-NO on an experimental model rat transplantation. Methods Orthotopic was performed syngeneic (LEW LEW) rats. Liver determined by measuring serum function tests 6 48 hours In some experiments, rats received L-arginine supplementation 0 24 other synthase inhibitors (L-NAME or L-NIL) were injected at time isograft Results L-Arginine decreased hepatic transaminase levels all points examined (6-48 hours). produced a significant improvement 12 reperfusion. inhibitor L-NAME caused increase injection. inducible (iNOS)-specific L-NIL had no effect Conclusions show improve have protective transplanted graft. may be mediated low-level cNOS-derived NO.