S-nitroso-N-acetylcysteine: a promising drug for early ischemia/reperfusion injury in rat liver.

摘要: Abstract Background/Aims Ischemia-reperfusion (I/R) injury is among the major causes of poor graft function early after liver transplantation that adversely influences patient survival. A variety mediators have been implicated in pathogenesis I/R vascular injury, including nitric oxide (NO). Because beneficial effects NO during preconditioning and reperfusion, strategies to prevent or ameliorate through stimulation hepatic production are an area significant clinical interest. We evaluated role S-nitroso-N-acetylcysteine (SNAC) as donor prevention animal model. Methods Adult male Wistar rats were randomly assigned 3 experimental groups containing 5 animals each: University Wisconsin (UW) solution group; SNAC SNAC-containing UW (SNAC+UW) group. Aspartate aminotransferase (AST), alanine (ALT) lactate dehydrogenase (LDH) determined samples cold storage at 2, 4, 6 hours preservation. After storage, applied a 15-minute reperfusion period. Thereafter, was interrupted with blood obtained measure AST, ALT, LDH, thiobarbituric acid reactive substances (TBARS). Hepatic fragments processed for histologic analysis, determine TBARS, catalase, glutathione levels. Results During preservation, AST LDH significantly lower than group SNAC+UW (P = .004 P .03, respectively). ALT comparable .3). serum levels well TBARS catalase showed no differences groups. Glutathione concentration Conclusion The greater protective effect preserve rat livers but it UW.