Pharmacological preconditioning using intraportal infusion of L-arginine protects against hepatic ischemia reperfusion injury.

摘要: Objective The present study examined the effects of intraportal infusion L-arginine on ischemia/reperfusion injury (I/RI) in pig livers, by observing changes liver function, cell morphology, and mitochondrial ultrastructure. Background involvement nitric oxide (NO) pathway reperfusion-ischemic phenomenon is complex not fully understood. Likewise, little known about possible benefit (substrate for NO synthesis) I/RI. Methods A model consisting 90 min hepatic ischemia 180 reperfusion was employed. Eighteen female hybrid pigs were randomly divided into three groups: sham-operated, non-preconditioned, pharmacologically preconditioned group (intraportal 400 mg/kg) 10 before being subjected to reperfusion. Serum concentrations aspartate aminotransferase (AST), alanine (ALT), thiobarbituric acid reactive substances (TBARS), bile flow measured. Liver biopsies taken after histology, caspase-3 immunohistochemistry, ultrastructural examination mitochondria. Results In group, we observed increased ( P = 0.02) more evident non-preconditioned than sham operated group. Infiltrating PMNs 0.01) showed an approximately 2.5-fold decrease activity relative Conclusions Pharmacological preconditioning with provided protection against I/RI early phases period. mechanisms underlying protective effect may include preservation structure inhibition activity.