Genetically engineered theranostic mesenchymal stem cells for the evaluation of the anticancer efficacy of enzyme/prodrug systems
作者： Faranak Salman Nouri , Xing Wang , Arash Hatefi
DOI: 10.1016/J.JCONREL.2015.01.003
关键词: Pharmacology 、 Mesenchymal stem cell 、 Cytosine deaminase 、 Biology 、 Suicide gene 、 Thymidine kinase 、 Uracil phosphoribosyltransferase 、 Viral vector 、 Prodrug 、 Genetically modified organism
摘要: Over the past decade, various enzyme/prodrug systems such as thymidine kinase/ganciclovir (TK/GCV), yeast cytosine deaminase/5-fluorocytosine (yCD/5-FC) and nitroreductase/CB1954 (NTR/CB1954) have been used for stem cell mediated suicide gene therapy of cancer. Yet, no study has conducted to compare demonstrate advantages disadvantages using one system over another. Knowing that each its own strengths weaknesses, we utilized mesenchymal cells (MSCs) a medium perform first time comparative illustrated impact subtle differences among these on therapeutic outcome. For purposes, genetically modified MSCs stably express panel four genes including TK (TK007 TK(SR39) mutants), deaminase:uracil phosphoribosyltransferase (yCD:UPRT) nitroreductase (NTR). Then, evaluated anticancer efficacies engineered in vitro vivo by SKOV3 line which is sensitive all systems. In addition, were luciferase making them suitable quantitative imaging dose-response relationship studies animals. Considering limitations imposed prodrugs' bystander effects, our findings show yCD:UPRT/5-FC most effective ones tested. Our also theranostic are reliable side-by-side evaluation screening at preclinical level. The results this could help scientists who utilize cell-based, non-viral or viral vectors cancer make more informed decisions when choosing
elsevier.com
sci-hub.se 参考文章(38)
Zahra Karjoo, Vidya Ganapathy, Arash Hatefi, Gene-Directed Enzyme Prodrug Cancer Therapy Gene Therapy of Cancer (Third Edition). pp. 77- 91 ,(2013) , 10.1016/B978-0-12-394295-1.00006-8
F. Garcia-Sanchez, G. Pizzorno, S.Q. Fu, T. Nanakorn, D.S. Krause, J. Liang, E. Adams, J.J. Leffert, L.H. Yin, M.R. Cooperberg, E. Hanania, W.L. Wang, J.H. Won, X.Y. Peng, R. Cote, R. Brown, B. Burtness, R. Giles, R. Crystal, A.B. Deisseroth, Cytosine deaminase adenoviral vector and 5-fluorocytosine selectively reduce breast cancer cells 1 million-fold when they contaminate hematopoietic cells: A potential purging method for autologous transplantation Blood. ,vol. 92, pp. 672- 682 ,(1998) , 10.1182/BLOOD.V92.2.672
Dong Wei, Gong Chen, De-zhi Shi, Pei-yuan Gu, Wei-xing Hu, Li-xin Li, Pharmacokinetics and the bystander effect in CD::UPRT/5-FC bi-gene therapy of glioma. Chinese Medical Journal. ,vol. 122, pp. 1267- 1272 ,(2009)
Mark S. Kokoris, Margaret E. Black, Peter Sabo, Herpes Simplex Virus-1 Thymidine Kinase Mutants Created by Semi-Random Sequence Mutagenesis Improve Prodrug-mediated Tumor Cell Killing Cancer Research. ,vol. 61, pp. 3022- 3026 ,(2001)
Uwe M. Fischer, Matthew T. Harting, Fernando Jimenez, Werner O. Monzon-Posadas, Hasen Xue, Sean I. Savitz, Glen A. Laine, Charles S. Cox, Pulmonary Passage is a Major Obstacle for Intravenous Stem Cell Delivery: The Pulmonary First-Pass Effect Stem Cells and Development. ,vol. 18, pp. 683- 692 ,(2009) , 10.1089/SCD.2008.0253
Jianyong Xiao, Guangxian Zhang, Pengxiang Qiu, Xijuan Liu, Yingya Wu, Biaoyan Du, Jiefen Li, Jing Zhou, Jingjing Li, Yuhui Tan, Tanshinone IIA Increases the Bystander Effect of Herpes Simplex Virus Thymidine Kinase/Ganciclovir Gene Therapy via Enhanced Gap Junctional Intercellular Communication PLoS ONE. ,vol. 8, pp. e67662- ,(2013) , 10.1371/JOURNAL.PONE.0067662
Lucia Kucerova, Miroslava Matuskova, Andrea Pastorakova, Silvia Tyciakova, Jana Jakubikova, Roman Bohovic, Veronika Altanerova, Cestmir Altaner, Cytosine deaminase expressing human mesenchymal stem cells mediated tumour regression in melanoma bearing mice The Journal of Gene Medicine. ,vol. 10, pp. 1071- 1082 ,(2008) , 10.1002/JGM.1239
C Richard, W Duivenvoorden, D Bourbeau, B Massie, W Roa, J Yau, J Th'ng, Sensitivity of 5-fluorouracil-resistant cancer cells to adenovirus suicide gene therapy Cancer Gene Therapy. ,vol. 14, pp. 57- 65 ,(2007) , 10.1038/SJ.CGT.7700980
H Kosaka, T Ichikawa, K Kurozumi, H Kambara, S Inoue, T Maruo, K Nakamura, H Hamada, I Date, Therapeutic effect of suicide gene-transferred mesenchymal stem cells in a rat model of glioma. Cancer Gene Therapy. ,vol. 19, pp. 572- 578 ,(2012) , 10.1038/CGT.2012.35
C L White, T Menghistu, K R Twigger, P F Searle, S A Bhide, R G Vile, A A Melcher, H S Pandha, K J Harrington, Escherichia coli nitroreductase plus CB1954 enhances the effect of radiotherapy in vitro and in vivo Gene Therapy. ,vol. 15, pp. 424- 433 ,(2008) , 10.1038/SJ.GT.3303081