Transplantation of polymer-encapsulated fetal hippocampal cells into ischemic lesions of adult rat hippocampus

摘要: Abstract In a previous study we demonstrated that fetal hippocampal cells, when transplanted into ischemic lesions of the adult rat hippocampus, can survive in large numbers host brain and show innervation transplants by cholinergic fibers originated from brain. The present was undertaken an attempt to elucidate hypothesis fiber connections forming synapses between neurons play important role survival cells. We polymer-encapsulated cells prepared E17–18 fetuses hippocampus at which CA1 pyramidal selectively died, examined both histochemically or immunohistochemically for their expression synaptic vesicle protein, synaptophysin, dendritic cytoskeltal microtubule-associated protein 2 (MAP 2) within them. addition, were acetylcholine esterase (AChE) histochemistry. results polymerencapsulated could brain; however, number surviving markedly decreased following transplantation, whereas no host-derived penetrated polymer membrane capsules transplantation. cluster only slight synaptophysin extensive growth dendrites detected. indicate direct contact tissue transplant such allografted neurons.