Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor
作者： H. Modjtahedi , S. A. Eccles , G. Box , J. Styles , C. J. Dean
DOI: 10.1007/BF03033871
关键词: Epitope 、 Cancer research 、 Transforming growth factor 、 Immunology 、 Biology 、 Antibody 、 Receptor 、 Epidermal growth factor 、 In vitro 、 Epidermal growth factor receptor 、 Monoclonal antibody 、 Biophysics 、 Cell biology
摘要: The receptor (EGFR) for epidermal growth factor (EGF) and transforming alpha (TGFα) is often overexpressed in certain types of human malignancy high levels expression the and/ or coexpression factors. EGF TGFα have also been correlated with poor prognosis many patients. We produced a number rat monoclonal antibodies (MAbs) against four distinct epitopes on external domain are currently evaluating their potential therapeutic use. Nine these MAbs were found to inhibit binding TGF tumor cells able vitro vivo that overexpress receptor. Here, we describe results experiments determine antitumor activity combination treatment two directed separate EGFR. Our showed xenografts anti-EGFR bind was not as effective ICR62 alone, which binds epitope C EGFR IgG2b isotype. A phase I clinical trial antibody underway Royal Marseden Hospital (UK) patients head neck lung carcinomas.
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sci-hub.se 参考文章(32)
John Mendelsohn, Anti-EGF receptor monoclonal antibodies: biological studies and potential clinical applications. Transactions of the American Clinical and Climatological Association. ,vol. 100, pp. 31- 38 ,(1989)
MJ Dyer, G Hale, FG Hayhoe, H Waldmann, Effects of CAMPATH-1 antibodies in vivo in patients with lymphoid malignancies: influence of antibody isotype. Blood. ,vol. 73, pp. 1431- 1439 ,(1989) , 10.1182/BLOOD.V73.6.1431.1431
L A Gyure, S M Hobbs, C J Dean, J M Styles, J G Hall, J Peppard, E Jackson, The production of hybridomas from the gut associated lymphoid tissue of tumour bearing rats. I. Mesenteric nodes as a source of IgG producing cells. Clinical and Experimental Immunology. ,vol. 57, pp. 358- 364 ,(1984)
J. Denry Sato, Anh D. Le, Tomoyuki Kawamoto, [6] Derivation and assay of biological effects of monoclonal antibodies to epidermal growth factor receptors Methods in Enzymology. ,vol. 146, pp. 63- 81 ,(1987) , 10.1016/S0076-6879(87)46009-6
S. Cohen, H. Ushiro, C. Stoscheck, M. Chinkers, A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles. Journal of Biological Chemistry. ,vol. 257, pp. 1523- 1531 ,(1982) , 10.1016/S0021-9258(19)68224-4
Stephen L. Briggs, Magda C. Gutowski, David A. Johnson, Epidermal growth factor receptor-reactive monoclonal antibodies: xenograft antitumor activity alone and as drug immunoconjugates. Cancer Research. ,vol. 51, pp. 5471- 5475 ,(1991)
M. Das, T. Miyakawa, C. F. Fox, R. M. Pruss, A. Aharonov, H. R. Herschman, Specific radiolabeling of a cell surface receptor for epidermal growth factor. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 2790- 2794 ,(1977) , 10.1073/PNAS.74.7.2790
E Jaros, RH Perry, L Adam, PJ Kelly, PJ Crawford, RM Kalbag, AD Mendelow, RP Sengupta, ADJ Pearson, Prognostic implications of p53 protein, epidermal growth factor receptor, and Ki-67 labelling in brain tumours. British Journal of Cancer. ,vol. 66, pp. 373- 385 ,(1992) , 10.1038/BJC.1992.273
H Modjtahedi, S Eccles, G Box, J Styles, C Dean, Immunotherapy of human tumour xenografts overexpressing the EGF receptor with rat antibodies that block growth factor-receptor interaction British Journal of Cancer. ,vol. 67, pp. 254- 261 ,(1993) , 10.1038/BJC.1993.49
GRAHAM CARPENTER, LLOYD KING, STANLEY COHEN, Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro. Nature. ,vol. 276, pp. 409- 410 ,(1978) , 10.1038/276409A0