Detection of tumor-associated antigens in sera of canine cancer patients by monoclonal antibodies generated against canine mammary carcinoma cells
作者： Jianyi Wang , Cindy J. Brunner , Aniruddha Gangopadhyay , Allison Church Bird , Lauren G. Wolfe
DOI: 10.1016/0165-2427(95)05436-A
关键词: Biology 、 Antibody 、 Carcinoma 、 Cell culture 、 Antigen 、 Monoclonal antibody 、 Canine Mammary Carcinoma 、 Pathology 、 Sarcoma 、 Mammary tumor 、 Molecular biology
摘要: Two murine monoclonal antibodies (MAbs), 1A10 and SB2, generated against a canine mammary carcinoma cell line, were used in competitive enzyme-linked immunosorbent assay (ELISA) to measure tumor-associated antigens (TAAs) serum samples. Sera tested from disease-free dogs diagnosed with carcinoma, non-mammary sarcoma, benign tumor, or non-neoplastic disease. Serum antigen concentrations measured by ELISA expressed as inhibitory units (IU). The upper limit of normal, defined the mean plus 2 SD TAA concentration dogs, was 20 IU antibody 22 SB2. Compared frequency TAA-positive sera significantly greater (P < 0.05) among when MAbs also sarcoma MAb Testing sample both rather than just one increased sensitivity for detection. presence might serve useful marker certain types carcinomas sarcomas cancer patients.
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sci-hub.se 参考文章(49)
J.L. Jensen, G.D. Maclean, M.R. Suresh, A. Almeida, D. Jette, S. Lloyd, D. Bodnar, M. Krantz, B.M. Longenecker, Possible utility of serum determinations of CA 125 and CA 27.29 in breast cancer management. International Journal of Biological Markers. ,vol. 6, pp. 1- 6 ,(1991) , 10.1177/172460089100600101
K. A. Wightman, W. W. Carlton, George Sandusky, Immunocytochemical study of tissues from clinically normal dogs and of neoplasms, using keratin monoclonal antibodies. American Journal of Veterinary Research. ,vol. 52, pp. 613- 618 ,(1991)
Serological cancer markers Humana Press. ,(1992) , 10.1007/978-1-4612-0401-5
Jack E. Moulton, Tumors in Domestic Animals ,(1978)
Steven A. Stacker, Joe Tjandra, James Bishop, Nigel P. M. Sacks, Christopher H. Thompson, Ian F. C. McKenzie, Melinda G. Lowe, Detection of mammary serum antigen in sera from breast cancer patients using monoclonal antibody 3E1.2 Cancer Research. ,vol. 48, pp. 7060- 7066 ,(1988)
David Lane, Ed Harlow, Antibodies: A Laboratory Manual ,(1988)
Glenn Y. Terashita, Paul I. Terasaki, Jane Galton, Masaki Hirota, Kiyoyasu Fukushima, Masaaki Kawahara, Detection of tumor-associated antigens in the sera of lung cancer patients by three monoclonal antibodies. Cancer Research. ,vol. 45, pp. 6453- 6456 ,(1985)
J L Magnani, B Nilsson, M Brockhaus, D Zopf, Z Steplewski, H Koprowski, V Ginsburg, A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II. Journal of Biological Chemistry. ,vol. 257, pp. 14365- 14369 ,(1982) , 10.1016/S0021-9258(19)45389-1
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
E. Hellmén, A. Lindgren, The expression of intermediate filaments in canine mammary glands and their tumors. Veterinary Pathology. ,vol. 26, pp. 420- 428 ,(1989) , 10.1177/030098588902600507