Ectopic expression of EFFECTOR OF TRANSCRIPTION perturbs gibberellin-mediated plant developmental processes.

摘要: The plant hormone gibberellin (GA) is known to modulate various aspects of cell differentiation and development. current model GA-mediated regulation based on a de-repressible system includes specific protein modification degradation. HRT, zinc finger from barley has been shown have GA-dependent transcriptional repressing activity the seed-specific α-amylase promoter [Raventos, D., Skriver, K., Schlein, M., Karnahl, Rogers, S.W., J.C. Mundy, J. 1998. J.␣Biol. Chem. 273: 23313–23320]. Here we report characterization dicot homologue Brassica napus (BnET) provide evidence for its role in GA response modulation suggesting that this could be conserved feature gene family. When BnET ectopically expressed either Arabidopsis or tobacco phenotypes include dwarfism due shorter internodes late flowering, reduced germination rate, increased anthocyanin content xylem lignification as marker terminal differentiation. Transient expression protoplasts supports notion most likely repression controlled genes. Finally, histological analysis showed contrast other deficient mutants were fewer but not smaller cells, function division control.