Emergence of the Asian 1 Genotype of Dengue Virus Serotype 2 in Viet Nam: In Vivo Fitness Advantage and Lineage Replacement in South-East Asia

摘要: A better description of the extent and structure genetic diversity in dengue virus (DENV) endemic settings is central to its eventual control. To this end we determined complete coding region sequence 187 DENV-2 genomes 68 E genes from viruses sampled Vietnamese patients between 1995 2009. Strikingly, an episode genotype replacement was observed, with Asian 1 lineage entirely displacing previously dominant Asian/American viruses. This event also seems have occurred within Thailand Cambodia, suggestive a major difference viral fitness. determine cause evolutionary compared both infectivity genotypes mosquitoes their viraemia levels humans. Although there little mosquitoes, observed significantly higher plasma paediatric infected relative viruses, phenotype that predicted result probability human-to-mosquito transmission. These results provide mechanistic basis marked change more broadly underscore understanding DENV dynamics can inform development vaccines anti-viral drugs.