EFFECT OF GLUCOCORTICOIDS IN VIVO AND IN VITRO ON NET GLUCOSE UPTAKE AND AMINO ACID INCORPORATION BY RAT-THYMUS CELLS.

摘要: Abstract 1. The general hypothesis that glucocorticoids may act on certain extrahepatic tissues via a direct, rapid inhibition of glucose utilization, has been tested using incubated suspensions rat-thymus cells. 2. It found net uptake by cells obtained from rats injected 2 h previously with cortisol (approx. 1 mg per 100 g rat wt.) is significantly lower than control 3. Cortisol in vitro, at concentrations down to 10−7M, decreases such cell preparations during the first hour incubation, and 10−6M (the only concentration tested) incorporation leucine into protein second hour. Deoxycorticosterone less active respect both amino acid incorporation. 4. These findings are taken show physiological action decrease thymus tissue. 5. From results incubations various glucose, it appears produced not simply consequence decreased availability cell.