Differentiation of Leptospira interrogans isolates by IS1500 hybridization and PCR assays.

摘要: Genetic variability among Leptospira interrogans (sensu stricto) serovars was assessed by Southern blot hybridization and PCR analyses. The experiments used probes directed to sequences in a recently described insertion element, IS1500. Hybridization analysis showed that IS1500 present on polymorphic fragments differences these patterns could be identify serovars. also useful discriminating between serovar pomona type kennewicki isolates, making possible the identification of 15 previously unrecognized genetic groups. A assay developed which primers are positioned near terminal inverted repeats element outward. This yielded characteristic amplification from allowing them identified. We applied assays several new animal isolates L. Nicaragua, had an outbreak human leptospirosis. Three groups were identified: one strain two genetically distinct may intermediates canicola portlandvere. IS-based typing should for epidemiological