Interactions between IL-4, anti-CD40, and anti-immunoglobulin as activators of locomotion of human B cells.

摘要: The locomotor properties of small, surface IgM+ and IgD+ B cells from the human tonsil were studied using polarization assays collagen gel invasion assays. These gave poor responses when freshly isolated tonsil; but 30 to 40% polarized invaded gels after overnight culture in IL-4 or anti-CD40. IL-13 had a similar weaker effect. Culture with anti-CD40 together higher proportion than either alone, anti-CD40, IL-4, anti-IgM still (> 60% polarized). Polarization increased gradually, during hours culture, unlike typical rapid response chemoattractants. We also immediate (< min) effects chemoattractants on cell polarization. cultured then washed, not fresh tonsil, immediately Similar anti-IgD, both pre- postculture observed, was stronger. IL-4-cultured incorporating anti-IgM, numbers control gels. Most invading IgM+. results suggest that activation requires two steps. capacity for locomotion is growth-related first activated by enhanced presence anti-IgM. Following activation, respond rapidly such as anti-Ig