Signal Transduction Involved in Cell Cycle Regulation of Normal Hepatocyte and Hepatoma Cells

摘要: The cell cycle is controlled by cyclin/cyclin-dependent kinase (CDK) complexes and CDK inhibitors. expression function of these molecules are induced modulated signal transduction governing growth differentiation. We found that when normal rat hepatocyte was factor (HGF), HGF receptor (MET) activated, with association MET receptor-bound protein (GRB)-2, which the initial step activation mitogen-activated (MAPK) cascade. also report MAPK cascade sequentially followed growth. Before density reached confluence, p21/WAF1, a inhibitor, prevented Cdk4 activity, resulting in arrest. Thus, growth, strictly controlled. In contrast, human hepatocellular carcinomas (HCCs), MAPK/ERK (extracellular regulated kinase) constitutively upregulated. Enhanced GRB-2 receptors or S0S, ras activator, observed, may account for activation. On other hand, p21/WAF1 HCCs deficient overwhelmed CDKs excess stoichiometry. These observations indicate active and/or disturbance regulators contributes much to unrestricted HCCs. Finally, we introduced deletion mutants into HepG2 cells, reduction activity prevention addition, vector arrested at G1/S transition inhibition results provide new strategy anticancer therapy modulating machinery.