Identification of the double-bond position in fatty acid methyl esters by liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry
作者： Vladimír Vrkoslav , Josef Cvačka
DOI: 10.1016/J.CHROMA.2012.04.055
关键词: Adduct 、 Atmospheric-pressure chemical ionization 、 Fatty acid 、 High-performance liquid chromatography 、 Mass spectrometry 、 Double bond 、 Chromatography 、 Acetonitrile 、 Analytical chemistry 、 Fragmentation (mass spectrometry) 、 Chemistry
摘要: Abstract Fatty acid methyl esters (FAMEs) were analysed by reversed-phase HPLC coupled with atmospheric pressure chemical ionisation (APCI) mass spectrometry. The chromatographic separations of the FAMEs optimised using acetonitrile or binary gradients and C18 C30 columns. gas-phase reactions unsaturated in APCI source provided [M+C 3 H 5 N] + adducts. When fragmented, these adducts yielded diagnostic ions, allowing unambiguous localisation double bonds. formation fragmentation acetonitrile-related adduct was utilised for structural characterisation separated HPLC. APCI-MS detection encompassed a full-spectrum scan (providing information on number carbons bonds) data-dependent MS/MS ions (the position bonds). utility this approach demonstrated mixture from blackcurrant-seed oil. All fatty acids known to exist sample correctly identified several others newly discovered. In terms sensitivity, HPLC/APCI-MS appeared be comparable GC/EI-MS.
elsevier.com
sci-hub.se 参考文章(29)
Xianlin Han, William W. Christie, Lipid Analysis: Isolation, Separation, Identification and Lipidomic Analysis ,(2010)
Michal Holčapek, Pavel Jandera, Jan Fischer, Bořivoj Prokeš, Analytical monitoring of the production of biodiesel by high-performance liquid chromatography with various detection methods. Journal of Chromatography A. ,vol. 858, pp. 13- 31 ,(1999) , 10.1016/S0021-9673(99)00790-6
Miroslav Lísa, Michal Holčapek, Triacylglycerols profiling in plant oils important in food industry, dietetics and cosmetics using high-performance liquid chromatography–atmospheric pressure chemical ionization mass spectrometry Journal of Chromatography A. ,vol. 1198-1199, pp. 115- 130 ,(2008) , 10.1016/J.CHROMA.2008.05.037
Vladimír Vrkoslav, Klára Urbanová, Josef Cvačka, Analysis of wax ester molecular species by high performance liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry. Journal of Chromatography A. ,vol. 1217, pp. 4184- 4194 ,(2010) , 10.1016/J.CHROMA.2009.12.048
Karel Stránský, Marie Zarevúcka, Zdeněk Wimmer, Gas chromatography analysis of blackcurrant oil in relation to its stability Food Chemistry. ,vol. 92, pp. 569- 573 ,(2005) , 10.1016/J.FOODCHEM.2004.10.010
Maged P. Mansour, Reversed-phase high-performance liquid chromatography purification of methyl esters of C16–C28 polyunsaturated fatty acids in microalgae, including octacosaoctaenoic acid [28:8(n-3)] Journal of Chromatography A. ,vol. 1097, pp. 54- 58 ,(2005) , 10.1016/J.CHROMA.2005.08.011
R.O Adlof, Separation of conjugated linoleic acid methyl esters by silver-ion high performance liquid chromatography in semi-preparative mode. Journal of Chromatography A. ,vol. 1033, pp. 369- 371 ,(2004) , 10.1016/J.CHROMA.2003.01.001
Colleen K. Pelt, Barry K. Carpenter, J. Thomas Brenna, Studies of structure and mechanism in acetonitrile chemical ionization tandem mass spectrometry of polyunsaturated fatty acid methyl esters Journal of the American Society for Mass Spectrometry. ,vol. 10, pp. 1253- 1262 ,(1999) , 10.1016/S1044-0305(99)00109-9
Alejandro Ruiz-Rodriguez, Guillermo Reglero, Elena Ibañez, Recent trends in the advanced analysis of bioactive fatty acids Journal of Pharmaceutical and Biomedical Analysis. ,vol. 51, pp. 305- 326 ,(2010) , 10.1016/J.JPBA.2009.05.012
Neil J. Oldham, Aleš Svatoš, Determination of the double bond position in functionalized monoenes by chemical ionization ion-trap mass spectrometry using acetonitrile as a reagent gas Rapid Communications in Mass Spectrometry. ,vol. 13, pp. 331- 336 ,(1999) , 10.1002/(SICI)1097-0231(19990315)13:5<331::AID-RCM487>3.0.CO;2-A