Comparison of Diafiltration and Tangential Flow Filtration for Purification of Nanoparticle Suspensions

摘要: The study reports evaluation of different purification processes for removing surplus surfactant and formulating stable nanoparticle dispersions. Nanoparticle formulations prepared from poly(d,l-lactide-co-glycolide) polyvinyl alcohol (PVA) were purified by a diafiltration centrifugal device (DCD), using 300K 100K molecular weight cut-off (MWCO) membranes tangential flow filtration (TFF) system with MWCO membrane. effects process parameters including MWCO, transmembrane pressure (TMP), mode TFF on evaluated, two techniques compared to the commonly used ultracentrifugation technique. Both DCD systems (concentration at TMP 10 psi) membrane removed maximal percent PVA dispersions (89.0 90.7%, respectively). T 90, time taken remove 90% in 200-ml sample, however, was considerably (9.6 2.8 h, Purified free aggregation ambient conditions over 3 days. This is contrast technique, which, although it can yield highly suffers drawbacks level irreversible loss fine particles supernatant during centrifugation. TFF, concentration 10 psi, relatively quick, efficient, cost-effective technique large batch (≥200 ml). be an alternative method small volumes.