Mitochondrial and nucleolar localization of cysteine desulfurase Nfs and the scaffold protein Isu in Trypanosoma brucei.

摘要: Trypanosoma brucei has a complex life cycle during which its single mitochondrion is subjected to major metabolic and morphological changes. While the procyclic stage (PS) of insect vector contains large reticulated mitochondrion, counterpart in bloodstream (BS) parasitizing mammals highly reduced seems be devoid most functions. We show here that key Fe-S cluster assembly proteins are still present active this organelle produced clusters incorporated into overexpressed enzymes. Importantly, cysteine desulfurase Nfs, equipped with nuclear localization signal, was detected nucleolus both T. stages. The scaffold protein Isu, an interacting partner also found have dual nucleolus, while frataxin ferredoxins confined mitochondrion. Moreover, upon depletion cytosolic tRNA thiolation dropped PS but not BS parasites.