Quantitative Studies of the Lipid Mediators of Inflammation Using Liquid Chromatography-Electrospray Mass Spectrometry

摘要: The lipid mediators of inflammation include diverse biologically active molecules, mainly eicosanoids, but also phospholipids that modulate functional responses inflammatory cells, i.e., adherence, locomotion, phagocytosis, production reactive oxygen species, release lysosomal enzymes, and synthesis cytokines other mediators. Lipid regulate important events the process, such as leukocyte trafficking, vascular permeability smooth muscle contractility (1–3). Thus, play roles in regulation process health, which they mediate immune response promote tissue repair, well diseases, dysregulation their biosynthesis and/or activity results damage deleterious effects. Among many mediators, PG E2 (PGE2), is produced either by constitutive cyclooxygenase (COX-1) or inducible (COX-2), plays an role phagocyte acts a potent vasodilator. Leukotriene B4 (LTB4) derived from 5-lipoxygenase (5-LO) pathway chemoattractant for phagocytes likely component body defense mechanisms against infection. LTB4 has been recently involved development collagen-induced arthritis mice (4), animal model rheumatoid arthritis. cysteinyl-LT (LTC4, D4, E4), 5-LO pathway, are stimuli have clearly implicated bronchospasm asthma (5). Lipoxin A4 (LXA4), synthesized through 12- 15-LO pathways, shown to act downregulator migration therefore believed be part complex network trafficking (6).