Imaging of nuclear factor κB activation induced by ionizing radiation in human embryonic kidney (HEK) cells.

摘要: Ionizing radiation modulates several signaling pathways resulting in transcription factor activation. Nuclear kappa B (NF-κB) is one of the most important factors that respond to changes environment a mammalian cell. NF-κB plays key role not only inflammation and immune regulation but also cellular response. In response DNA damage, might inhibit apoptosis promote carcinogenesis. Our previous studies showed ionizing very effective inducing biological damages. Therefore, it understand radiation-induced cascade. The current study aims improve existing cell-based reporter assays for activation by use DD-tdTomato which destabilized variant red fluorescent protein tdTomato. It demonstrated exposure recombinant human embryonic kidney cells (HEK/293 transfected with constructs containing binding sites its promoter) induces NF-κB-dependent expression. Using this assays, was monitored flow cytometry fluorescence microscopy. Activation canonical pathway found be quicker than genotoxin- stress-induced pathway. X-rays activate HEK dose-dependent manner, extent higher as compared camptothecin.