Lack of Involvement of Ataxia Telangiectasia Mutated (ATM) in Regulation of Nuclear Factor-κB (NF-κB) in Human Diploid Fibroblasts
作者： Richard S. Paules , Albert S. Baldwin , Rodney E. Shackelford , Brian P. Ashburner
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摘要: It has been suggested that the cellular response to exposure ionizing radiation involves activation of transcription factor nuclear factor-kappaB (NF-kappaB) and this is defective in cells from individuals with ataxia telangiectasia (AT). In one study, it was found SV40 large T-transformed derived a patient null for AT mutated (ATM) gene exhibited constitutive NF-kappaB those cells, inhibition by expression modified form IkappaBalpha led correction radiosensitivity associated phenotype [M. Jung et al., Science (Washington DC), 268: 1691-1621, 1995]. From data, played role phenotype. We show here normal diploid patients do not exhibit NF-kappaB. Furthermore, we provide data transformation process T antigen AT-/- leads aberrant responses. Our studies highlight importance using diploid, nontransformed vitro relevant whenever possible.
aacrjournals.org参考文章(23)
Adapa V. Prasad, Natarajan Mohan, Bysani Chandrasekar, Martin L. Meltz, Activation of Nuclear Factor κB in Human Lymphoblastoid Cells by Low-Dose Ionizing Radiation Radiation Research. ,vol. 138, pp. 367- 372 ,(1994) , 10.2307/3578685
Richard S. Paules, Michael A. Tainsky, Cynthia L. Innes, William K. Kaufmann, Thea D. Tlsty, Lawrence A. Donehower, Eleni N. Levedakou, Denise A. Galloway, Sandra J. Wilson, Nelson Rhodes, Defective G2 Checkpoint Function in Cells from Individuals with Familial Cancer Syndromes Cancer Research. ,vol. 55, pp. 1763- 1773 ,(1995)
Dominique Stoppa-Lyonnet, Ethel Moustacchi, Agnes Ridet, Eric Duchaud, Nicolas Janin, Filippo Rosselli, Deregulated Apoptosis in Ataxia Telangiectasia: Association with Clinical Stigmata and Radiosensitivity Cancer Research. ,vol. 56, pp. 1400- 1404 ,(1996)
Natarajan Mohan, Martin L. Meltz, Induction of nuclear factor κB after low-dose ionizing radiation involves a reactive oxygen intermediate signaling pathway Radiation Research. ,vol. 140, pp. 97- 104 ,(1994) , 10.2307/3578574
T. S. Finco, A. A. Beg, A. S. Baldwin, Inducible phosphorylation of I kappa B alpha is not sufficient for its dissociation from NF-kappa B and is inhibited by protease inhibitors Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 11884- 11888 ,(1994) , 10.1073/PNAS.91.25.11884
C. Wang, NF-B Antiapoptosis: Induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to Suppress Caspase-8 Activation Science. ,vol. 281, pp. 1680- 1683 ,(1998) , 10.1126/SCIENCE.281.5383.1680
Timothy S. Finco, Albert S. Baldwin, Mechanistic aspects of NF-κB regulation: The emerging role of phosphorylation and proteolysis Immunity. ,vol. 3, pp. 263- 272 ,(1995) , 10.1016/1074-7613(95)90112-4
M A Brach, R Hass, M L Sherman, H Gunji, R Weichselbaum, D Kufe, Ionizing radiation induces expression and binding activity of the nuclear factor kappa B. Journal of Clinical Investigation. ,vol. 88, pp. 691- 695 ,(1991) , 10.1172/JCI115354
M Jung, Y Zhang, S Lee, A Dritschilo, Correction of radiation sensitivity in ataxia telangiectasia cells by a truncated I kappa B-alpha Science. ,vol. 268, pp. 1619- 1621 ,(1995) , 10.1126/SCIENCE.7777860
N. Li, M. Karin, Ionizing radiation and short wavelength UV activate NF-kappaB through two distinct mechanisms. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 13012- 13017 ,(1998) , 10.1073/PNAS.95.22.13012