Differences in the ability to suppress interferon β production between allele A and allele B NS1 proteins from H10 influenza A viruses.

作者: Siamak Zohari , Muhammad Munir , Giorgi Metreveli , Sándor Belák , Mikael Berg

DOI: 10.1186/1743-422X-7-376

关键词: GeneticsAlleleGeneBiologyJAK-STAT signaling pathwayRegulation of gene expressionVirologyInfluenza A virusMinkMessenger RNAInterferon inducer

摘要: In our previous study concerning the genetic relationship among H10 avian influenza viruses with different pathogenicity in mink (Mustela vison), we found that these differences were related to amino acid variations NS1 protein. this study, extend work further investigate effect of from gene pools on type I IFN promoter activity, production IFN-β, as well expression IFN-β mRNA response poly I:C. Using a model system, first demonstrated A/mink/Sweden/84 (H10N4) (allele A) could suppress an interferon-stimulated element (ISRE) reporter system about 85%. The other B), A/chicken/Germany/N/49 (H10N7), was also able but only 20%. abilities two NS1s alleles ISRE clearly reflected by protein and expressions shown ELISA RT-PCR assays. These studies reveal non-structural 1 (NS1) viruses, one allele A another B, show interferon β expression. It has been hypothesised some because interactions inhibitions at later stages receptor, such JAK/STAT pathway. This might reflect additional effects immune evasion potential NS1s.

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