Double-stranded RNA-induced activation of activating protein-1 promoter is differentially regulated by the non-structural protein 1 of avian influenza A viruses.

摘要: Non-structural protein 1 (NS1) of influenza A viruses is a multifunctional that antagonizes the host immune response by interfering with several signaling pathways. Based on putative amino acid sequences, NS1 proteins are categorized into two gene pools, allele and B. Here we identified H6N8 H4N6 able to inhibit double-stranded RNA (dsRNA)-induced activating protein-1 (AP-1) promoter in cultured cell lines (human A549 mink lung cells). Allele B from corresponding subtypes weak this inhibition, despite significant levels expression each human cells. Furthermore, capability AP-1 was mapped effector domain, since binding domain alone lost its ability activation. Chimeric forms protein, composed either or B, showed comparable inhibition their wild-type proteins, proteins. Both alleles were expressed levels, localized predominantly nucleus These results underscore importance inhibiting activation, biological function stabilizing domain. Further, revealed versatile nature transcription factor, manner dependent type. Comprehensive studies, focusing molecular mechanisms behind differential may facilitate exploration zoonotic pathogenic potential viruses.