Assessment of essential fatty acid and ω3-fatty acid status by measurement of erythrocyte 20:3ω9 (Mead acid), 22:5ω6/20:4ω6 and 22:5ω6/22:6ω3

摘要: Abstract Background . Early suspicion of essential fatty acid deficiency (EFAD) or ω3-deficiency may rather focus on polyunsaturated (PUFA) long-chain PUFA (LCP) analyses than clinical symptoms. We determined cut-off values for biochemical EFAD, ω3-and ω3/22:6ω3 [docosahexaenoic (DHA)]-deficiency by measurement erythrocyte 20:3ω9 (Mead acid), 22:5ω6/20:4ω6 and 22:5ω6/22:6ω3, respectively. Methods Cut-off values, based 97.5 percentiles, derived from an apparently healthy omnivorous group (six Dominica breast-fed newborns, 32 27 formula+LCP-fed Dutch low-birth-weight infants, 31 Jerusalem 33 3.5-year-old 69 adults seven mothers) with low dietary LCP intake (81 formula-fed 12 vegans). were evaluated their application in EFAD suspected 108, mostly malnourished, Pakistani children, three pediatric patients chronic fat-malabsorption (abetal-ipoproteinemia, congenital jejunal biliary atresia) one patient a peroxisomal β-oxidation disorder. Results. Erythrocyte 20:3ω9, 22:5ω6/22:6ω3 proved age-dependent up to 0.2 years. ages above years were: 0.46mol% 0.068mol/mol ω3-deficiency, 0.22mol/mol ω3/DHA-marginality 0.48mol/mol ω3/DHA-deficiency. Use RBC identified 20.4% the subjects as EFAD+ω3-deficient, 12.9% EFAD+ω3-sufficient, 38.9% EFA-sufficient+ω3-deficient 27.8% EFA-sufficient+ω3-sufficient. The disorder was classified EFA-sufficient, ω3-sufficient (based 22:5ω6/20:4ω6) ω3/DHA-deficient 22:5ω6/22:6ω3). other ω3-deficient ω3/DHA-deficient. Conclusion. combination present EFA, ω3 ω3/DHA status assessment, serve supplement intervention until better concepts have emerged.