Imaging developing neural morphology using optical coherence tomography

摘要: Imaging technologies offer numerous possibilities to investigate the processes involved in neural development. The optical coherence tomography (OCT) technology is analogous ultrasound backscatter microscopy except reflections of light are detected rather than sound. OCT combines high-resolution vivo imaging a diode-based benchtop instrument capable micron-scale resolution transparent and non-transparent biological specimens. In this paper, we examine potential using for investigation developing morphology. To demonstrate capabilities technique assessing development, have chosen image early normal abnormal morphology common developmental biology model, Xenopus laevis. images clearly identify gross subtle differences structure may an alternative costly time-consuming process repeated histological preparation studies. Because can be performed rapidly repeatedly, morphological changes single specimens followed throughout illustrate future technique, state-of-the-art Cr4+:forsterite modelocked laser used as broad bandwidth source individual cells specimen.