The concentration of circulating corticotropin-releasing hormone mRNA in maternal plasma is increased in preeclampsia.
作者： Rossa W K Chiu , Y M Dennis Lo , Enders K O Ng , Tse N Leung , Nancy B Y Tsui
DOI: 10.1373/49.5.727
关键词: Hormone 、 Pregnancy 、 Corticotropin-releasing hormone 、 Fetus 、 Endocrinology 、 Biology 、 Preeclampsia 、 Internal medicine 、 Postpartum period 、 Gestational age 、 Blood plasma
摘要: Background: Increased fetal DNA in maternal plasma/serum has been reported pregnancies complicated by preeclampsia. We hypothesize that RNA may also be increased plasma preeclampsia. Methods: developed a real-time quantitative reverse transcription-PCR assay to measure the concentration of mRNA corticotropin-releasing hormone ( CRH ) locus. Peripheral blood samples were obtained from healthy pregnant women both before and 2 h after delivery. suffering preeclampsia controls matched for gestational age. Plasma was harvested these samples, extracted. subjected analysis assay. Results: detected 10 third trimester. found cleared very rapidly cesarean section, with no detectable signal postpartum. concentrations 1070 102 copies/mL, respectively, 12 preeclamptic age (Mann–Whitney test, P <0.001). Conclusion: represents new molecular marker Maternal is gender- polymorphism-independent allow noninvasive gene-expression profiling an unborn fetus.
oup.com
core.ac.uk
aaccjnls.org
hku.hk参考文章(30)
YM Dennis Lo, Tse N Leung, Mark SC Tein, Ian L Sargent, Jun Zhang, Tze K Lau, Christopher J Haines, Christopher WG Redman, Quantitative Abnormalities of Fetal DNA in Maternal Serum in Preeclampsia Clinical Chemistry. ,vol. 45, pp. 184- 188 ,(1999) , 10.1093/CLINCHEM/45.2.184
Enders KO Ng, Nancy BY Tsui, Nicole YL Lam, Rossa WK Chiu, Simon CH Yu, SC Cesar Wong, Elena SF Lo, Timothy H Rainer, Philip J Johnson, YM Dennis Lo, Presence of Filterable and Nonfilterable mRNA in the Plasma of Cancer Patients and Healthy Individuals Clinical Chemistry. ,vol. 48, pp. 1212- 1217 ,(2002) , 10.1093/CLINCHEM/48.8.1212
Dorine W Swinkels, Jacques B de Kok, Jan CM Hendriks, Erwin Wiegerinck, Petra LM Zusterzeel, Eric AP Steegers, Hemolysis, Elevated Liver Enzymes, and Low Platelet Count (HELLP) Syndrome as a Complication of Preeclampsia in Pregnant Women Increases the Amount of Cell-free Fetal and Maternal DNA in Maternal Plasma and Serum Clinical Chemistry. ,vol. 48, pp. 650- 653 ,(2002) , 10.1093/CLINCHEM/48.4.650
Tai-Wah Lau, Tse N Leung, Lisa YS Chan, Tze K Lau, KC Allen Chan, Wing H Tam, YM Dennis Lo, None, Fetal DNA Clearance from Maternal Plasma Is Impaired in Preeclampsia Clinical Chemistry. ,vol. 48, pp. 2141- 2146 ,(2002) , 10.1093/CLINCHEM/48.12.2141
Mark McLean, Andrew Bisits, Joanne Davies, Russell Woods, Philip Lowry, Roger Smith, A placental clock controlling the length of human pregnancy Nature Medicine. ,vol. 1, pp. 460- 463 ,(1995) , 10.1038/NM0595-460
J.M Roberts, C.W.G Redman, Pre-eclampsia: more than pregnancy-induced hypertension The Lancet. ,vol. 341, pp. 1447- 1451 ,(1993) , 10.1016/0140-6736(93)90889-O
ROBIN S. GOLAND, SHARON L. WARDLAW, RAYMOND I STARK, LAWRENCE S. BROWN, ANDREW G. FRANTZ, High levels of corticotropin-releasing hormone immunoactivity in maternal and fetal plasma during pregnancy. The Journal of Clinical Endocrinology and Metabolism. ,vol. 63, pp. 1199- 1203 ,(1986) , 10.1210/JCEM-63-5-1199
Xiao Yan Zhong, Martin R. B�rk, Carolyn Troeger, Laird R. Jackson, Wolfgang Holzgreve, Sinuhe Hahn, Fetal DNA in maternal plasma is elevated in pregnancies with aneuploid fetuses Prenatal Diagnosis. ,vol. 20, pp. 795- 798 ,(2000) , 10.1002/1097-0223(200010)20:10<795::AID-PD897>3.0.CO;2-P
Tamotsu Shibasaki, Emi Odagiri, Kazuo Shizume, Nicholas Ling, Corticotropin-releasing factor-like activity in human placental extracts The Journal of Clinical Endocrinology and Metabolism. ,vol. 55, pp. 384- 386 ,(1982) , 10.1210/JCEM-55-2-384
Xiao Yan Zhong, Hannele Laivuori, Jeffrey C. Livingston, Olavi Ylikorkala, Baha M. Sibai, Wolfgang Holzgreve, Sinuhe Hahn, Elevation of both maternal and fetal extracellular circulating deoxyribonucleic acid concentrations in the plasma of pregnant women with preeclampsia. American Journal of Obstetrics and Gynecology. ,vol. 184, pp. 414- 419 ,(2001) , 10.1067/MOB.2001.109594