Effects of n-3 PUFAs on Intestinal Mucosa Innate Immunity and Intestinal Microbiota in Mice after Hemorrhagic Shock Resuscitation

摘要: n-3 polyunsaturated fatty acids (PUFAs) can improve the function of intestinal barrier after damage from ischemia-reperfusion or hemorrhagic shock resuscitation (HSR). However, effects PUFAs on microbiota and innate immunity mucosa HSR remain unclear. In present study, 40 C57BL/6J mice were randomly assigned to five groups: control, sham, HSR, + n-6 PUFAs. Mice sacrificed 12 h HSR. Liver, spleen, mesenteric lymph nodes terminal ileal tissues collected. Intestinal mucosae scraped aseptically. Compared with group, number goblet cells increased, expression mucin 2 was restored disturbed partly stabilized in PUFA-administered groups, indicating that both reduced overproliferation Gammaproteobacteria while promoting growth Bacteroidetes. Notably, had an advantage over improving tissue levels lysozyme Thus, PUFAs, especially improved These findings suggest a clinical rationale for providing patients recovering ischemia-reperfusion.