Hypothetical protein Rv3423.1 of Mycobacterium tuberculosis is a histone acetyltransferase.
作者： Leny Jose , Ranjit Ramachandran , Raghu Bhagavat , Roshna Lawrence Gomez , Aneesh Chandran
DOI: 10.1111/FEBS.13566
关键词: Molecular biology 、 Biology 、 Mycobacterium smegmatis 、 Histone H3 、 Chromatin 、 Histone H1 、 Histone acetyltransferase 、 Mycobacterium tuberculosis 、 Histone 、 Hypothetical protein
摘要: We isolated an 8 kDa mycobacterial hypothetical protein, Rv3423.1, from the chromatin of human macrophages infected with Mycobacterium tuberculosis H37Rv. Bioinformatics predictions followed by in vitro biochemical assays purified recombinant protein showed that Rv3423.1 is a novel histone acetyltransferase acetylates H3 at K9/K14 positions. Transient transfection containing GFP-tagged H1 RFP-tagged revealed co-localizes nucleus. Co-immunoprecipitation confirmed Rv3423.1-histone interaction specific. was detected culture filtrate virulent but not avirulent M. tuberculosis. Infection smegmatis constitutively expressing resulted significant increase number intracellular bacteria. However, did seem to offer any growth advantage free-living smegmatis. It highly likely that, binding host chromatin, this may manipulate expression genes involved anti-inflammatory responses evade clearance and survive environment.
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