HIF-1A, pimonidazole, and iododeoxyuridine to estimate hypoxia and perfusion in human head-and-neck tumors.

摘要: PURPOSE: Tumor hypoxia measured by microelectrodes has been shown to indicate poor patient outcome. Here we investigated four potentially more widely applicable immunohistochemical parameters of tumor oxygenation and perfusion in human head-and-neck tumors. METHODS: Twenty patients with squamous cell carcinomas the head neck treated primary surgery were injected pimonidazole IdUrd evening before operation. Consecutive paraffin-embedded sections stained for blood vessels, pimonidazole, IdUrd, HIF-1alpha. labeling Ki-67 around individual vessels scored. The spatial relationship between HIF-1alpha was studied, as well distribution both markers a function distance from nearest vessel. RESULTS: Measurement all (diffusion-limited fraction, IdUrd-negative vessels) feasible, significant difference tumors found parameters. IdUrd-labeled cells absent some indicating lack perfusion, because these regions positive Ki-67. There correlation diffusion-limited fraction area images tumors, although no mean values per tumor. Colocalization low (0.02%-25%). Most expression profiles showed homogenous than pimonidazole. fractions 10 studied. CONCLUSIONS: Simultaneous measurements related are feasible (and easily applicable) resected different geographic distributions that might not be suitable marker chronic hypoxia. Each parameter will correlated outcome larger ongoing study on or without postoperative radiotherapy.