Fluorescent in situ hybridization and ex vivo 1H magnetic resonance spectroscopic examinations of meningioma tumor tissue

摘要: OBJECTIVE: Although histologically benign, Grade I meningiomas can sometimes behave aggressively. The clinically-aggressive subset of is typically indistinguishable from clinically-benign in vivo. We compared molecular genetic and biochemical findings to clinical, pathological, immunohistochemical information a series with identify characteristics that may be used distinguish between these two groups. METHODS: Tumor tissue samples 30 patients were harvested. Half the sample was embedded paraffin for fluorescent situ hybridization examine aberrations chromosomes 1p, 14q, 22q; other half snap frozen examined proton magnetic resonance spectroscopy concentrations key metabolites ex Clinical pathological parameters retrospectively reviewed as part routine clinical management. These data evaluated potential unique associations diagnostic significance. RESULTS: Molecular correlated behavior meningioma Specific chromosomal abnormalities aggressive phenotype: homogeneous loss presence any (P < 0.05). also influenced regrowth after subtotal resection. ratio choline glutamate histopathological subtype glutamine glutamate, glycine total creatine recurrence. Alanine decreased tumors recurred. CONCLUSION: Distinct alterations differentiated meningiomas.