TWO FORMS OF ADENOSINE DEAMINASE IN PIG EPIDERMIS
作者： Hajime Iizuka , Hiroko Koizumi , Katsuko Kamigaki , Takashi Aoyagi , Yusho Miura
DOI: 10.1111/J.1346-8138.1981.TB02573.X
关键词: Biochemistry 、 Column chromatography 、 Enzyme 、 Adenosine deaminase 、 Size-exclusion chromatography 、 Isoelectric point 、 Chemistry 、 Adenosine 、 Coformycin 、 Sephadex
摘要: Using pure pig skin epidermis, we investigated the biochemical nature of adenosine deaminase, Two different (large and small) forms deaminase were separated by a gel filtration technique using Sephadex G-150 column chromatography. The molecular weight large form was 300,000 to 350,000 that small 30,000 40,000. The kinetics two enzymes quite similar, in terms Km values for adenosine, substrate specificity, pH optima, temperature sensitivity, isoelectric points, inhibition coformycin, tight-binding inhibitor deaminase.
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