Protein-targeted corona phase molecular recognition
作者： Gili Bisker , Juyao Dong , Hoyoung D. Park , Nicole M. Iverson , Jiyoung Ahn
DOI: 10.1038/NCOMMS10241
关键词: Blood proteins 、 Molecular recognition 、 Nanotechnology 、 Fibrinogen 、 Macromolecule 、 Analyte 、 Chemistry 、 Biophysics 、 Quenching (fluorescence) 、 Protein Corona 、 Fibrinogen binding
摘要: Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by nanoparticle surface to recognize specific target analyte. This method has not yet been extended macromolecular analytes, including proteins. Herein we develop variant of CoPhMoRe screening procedure single-walled carbon nanotubes (SWCNT) use it against panel human blood proteins, revealing corona that recognizes fibrinogen with high selectivity. In response binding, SWCNT fluorescence decreases >80% at saturation. Sequential binding the three nodules is suggested selective quenching isolated sub-domains validated kinetics. The also occurs in serum environment, clinically relevant concentrations blood. These results open new avenues for synthetic, non-biological antibody analogues biological macromolecules, hold great promise medical clinical applications.
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