Monitoring mitochondrial membranes permeability in live neurons and mitochondrial swelling through electron microscopy analysis.
作者： Macarena S. Arrázola , Nibaldo C. Inestrosa
DOI: 10.1007/978-1-4939-2152-2_7
关键词: Chemistry 、 Mitochondrial permeability transition pore 、 Cell biology 、 Membrane 、 Live cell imaging 、 Calcein 、 Mitochondrial membrane transport protein 、 Mitochondrion 、 Inner mitochondrial membrane 、 Apoptosis
摘要: Maintenance of mitochondrial membrane integrity is essential for function and neuronal viability. Apoptotic stimulus or calcium overload leads to permeability transition pore (mPTP ) opening induces swelling, a common feature permeabilization. The first phenomenon can be evaluated in cells loaded with the dye calcein -AM quenched by cobalt, swelling detected electron microscopy through analysis integrity. Here, we describe live cell imaging assay detect development detailed morphological ultrastructural changes that mitochondria undergo during this process.
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