Transforming Growth Factor α (TGFα) Modulates the Effects of Genomic Imprinting and Prolongs Development of Parthenogenetic Mouse Embryos

摘要: The effect of transforming growth factor α (TGFα) on the development diploid parthenogenetic mouse embryos (CBA × C57BL/6)F1was studied. were in vitro treated with TGFα at morula stage. Upon reaching blastocyst stage, each embryo was implanted into uterus a pseudopregnant female. At dose 5 ng/ml, found to improve before implantation, increase significantly number developing blastocysts, and promote implantation uterus. After treatment 10 4% reached stage 30–45 somites had forelimb hindlimb buds; crown rump length size from vertex sacrum 2.0 3.8 mm. A well-developed placenta observed 6% TGFα-treated that somite stages. In most prominent (40–45 somites) ng/ml TGFα, placental diameter 4.0 4.2 mm day 12 gestation, which is close normal (fertilized) 11-day-old embryos. Our results suggest exogenous can modulate effects genomic imprinting improving formation trophoblast derivatives promoting longer postimplantation