Factors affecting success of PCR amplification of microsatellite loci from otter faeces

摘要: We investigated the effect of multiple variables on amplification success rate microsatellite DNA extracted from faeces wild Eurasian otters. The was affected by (i) type sample, with higher rates in anal jelly samples than faeces, and (ii) temperature, a negative increased temperature at time collection. To increase effectiveness genotyping otter we recommend collecting cold months early morning, preferably frozen state, collection samples, or part whenever possible.