Imprinted expression in cystic embryoid bodies shows an embryonic and not an extra-embryonic pattern

摘要: A large subset of mammalian imprinted genes show extra-embryonic lineage (EXEL) specific expression that is restricted to placental trophectoderm lineages and visceral yolk sac endoderm (ysE). Isolated ysE provides a homogenous in vivo model mid-gestation tissue examine the mechanism EXEL-specific gene silencing, but an vitro facilitate more rapid cost-effective experiments not available. Reports indicate ES cells differentiated into cystic embryoid bodies (EBs) contain ysE, so here we investigate if EBs expression. The pattern shown resemble fetal liver ysE. To reason for this characterized methylome transcriptome comparison by whole genome bisulphite sequencing RNA-seq. Cystic global hypermethylation low repeats, while shows hypomethylation high IAPEz retroviral as reported placenta. Transcriptome analysis confirmed are similar than express markers early embryonic endoderm. Genome-wide shares epigenetic repeat features with Contrary previous reports, do liver. This explains why reproduce seen embryo