Hif-1α regulates differentiation of limb bud mesenchyme and joint development
作者： Sylvain Provot , Dawn Zinyk , Yasemin Gunes , Richa Kathri , Quynh Le
关键词: Internal medicine 、 Cell biology 、 Limb bud 、 Endocrinology 、 Zone of polarizing activity 、 Mesenchyme 、 Endochondral ossification 、 Mesenchymal stem cell 、 Mesoderm 、 Biology 、 Chondrogenesis 、 Conditional gene knockout
摘要: Recent evidence suggests that low oxygen tension (hypoxia) may control fetal development and differentiation. A crucial mediator of the adaptive response cells to hypoxia is transcription factor Hif-1α. In this study, we provide mesenchymal condensations give origin endochondral bones are hypoxic during development, demonstrate Hif-1α expressed transcriptionally active in limb bud mesenchyme condensations. To investigate role early chondrogenesis, conditionally inactivated using a Prx1 promoter-driven Cre transgenic mouse. Conditional knockout does not impair condensation, but alters formation cartilaginous primordia. Late hypertrophic differentiation also affected as result delay chondrogenesis. addition, mutant mice show striking impairment joint development. Our study demonstrates crucial, previously unrecognized, chondrogenesis formation.
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