The migration and intermixing of donor and host glia on nitrocellulose polymers implanted into cortical lesion cavities in adult mice and rats.

摘要: The fate of neonatal glia (mostly glial fibrillary acidic protein-positive astrocytes), cultured on nitrocellulose papers and implanted into cortical lesion cavities, was examined in adult mice rats. In mice, a Y-chromosome-specific probe situ hybridization techniques were used to identify male cells. Male-female grafts allowed visualization donor their behaviour after transplantation; female-male an analysis how host cells responded the presence implants. There substantial intermixing cells, with many migrating away from implants onto both sides paper. rats, labelled fluorescein-conjugated latex microspheres prior transplantation polymers. rat data broadly consistent those obtained mouse; moreover, immunohistochemical studies rats suggested that majority previously cell-coated astrocytes. number studies, glia-coated polymers have been attempt promote regrowth axons across sites brain spinal cord. present work suggests transplanted may influence regenerative growth seen such