Cultured rat neuronal and glial cells entrapped within hydrogel polymer matrices: a potential tool for neural tissue replacement
作者： Stephane Woerly , Giles W. Plant , Alan R. Harvey
DOI: 10.1016/0304-3940(96)12349-1
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摘要: Cultured Schwann cells, neonatal astrocytes or cells dissociated from embryonic cerebral hemispheres were dispersed within poly-[N-(2-hydroxypropyl)-methacrylamide]-based hydrogel matrices by gel entrapment and maintained in vitro for 1-6 days. Glial pre-labelled with Hoechst 33342. Cell differentiation viability studied immunocytochemistry. Up to 15% of initially entrapped the polymer immunopositive low affinity nerve growth factor receptor, S100, glial fibrillary acidic protein (GFAP) laminin; up 10% GFAP laminin. Embryonic neurons displayed immunostaining neurofilaments. Hydrogels containing implanted into rat neocortex. These polymers supported cellular axonal ingrowth parts matrix.
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sci-hub.se 参考文章(14)
A.R. Harvey, Y. Fan, A.M. Connor, M.D. Grounds, M.W. Beilharz, The migration and intermixing of donor and host glia on nitrocellulose polymers implanted into cortical lesion cavities in adult mice and rats. International Journal of Developmental Neuroscience. ,vol. 11, pp. 569- 581 ,(1993) , 10.1016/0736-5748(93)90046-G
Kristine Lewandowska, Edward Pergament, Chaim N. Sukenik, Lloyd A. Culp, Cell‐Type‐Specific adhesion mechanisms mediated by fibronectin adsorbed to chemically derivatized substrata Journal of Biomedical Materials Research. ,vol. 26, pp. 1343- 1363 ,(1992) , 10.1002/JBM.820261007
Giles W. Plant, Alan R. Harvey, Traian V. Chirila, Axonal growth within poly (2-hydroxyethyl methacrylate) sponges infiltrated with Schwann cells and implanted into the lesioned rat optic tract. Brain Research. ,vol. 671, pp. 119- 130 ,(1995) , 10.1016/0006-8993(94)01312-6
S. Woerly, G. Maghami, R. Duncan, Poly(glyceryl methacrylate) hydrogels―effect of composition and crosslinking density on structure and release of dextran as a model macromolecule Journal of Bioactive and Compatible Polymers. ,vol. 7, pp. 305- 323 ,(1992) , 10.1177/088391159200700401
M.J Lydon, T.W Minett, B.J Tighe, Cellular interactions with synthetic polymer surfaces in culture Biomaterials. ,vol. 6, pp. 396- 402 ,(1985) , 10.1016/0142-9612(85)90100-0
Patrick Aebischer, Patrick A. Tresco, Shelley R. Winn, Lloyd A. Greene, Christine B. Jaeger, Long-term cross-species brain transplantation of a polymer-encapsulated dopamine-secreting cell line. Experimental Neurology. ,vol. 111, pp. 269- 275 ,(1991) , 10.1016/0014-4886(91)90093-R
Y. Sagot, S. A. Tan, E. Baetge, H. Schmalbruch, A. C. Kato, P. Aebischer, Polymer encapsulated cell lines genetically engineered to release ciliary neurotrophic factor can slow down progressive motor neuronopathy in the mouse. European Journal of Neuroscience. ,vol. 7, pp. 1313- 1322 ,(1995) , 10.1111/J.1460-9568.1995.TB01122.X
S. Woerly, G. Laroche, R. Marchand, J. Pato, V. Subr, K. Ulbrich, Intracerebral Implantation of Hydrogel-Coupled Adhesion Peptides: Tissue Reaction Neural Plasticity. ,vol. 5, pp. 245- 255 ,(1995) , 10.1155/NP.1994.245
Enoch Kim, Younan Xia, George M. Whitesides, Polymer microstructures formed by moulding in capillaries Nature. ,vol. 376, pp. 581- 584 ,(1995) , 10.1038/376581A0
B. Říhová, J. Kopeček, K. Ulbrich, V. Chytrý, Immunogenicity of N-(2-hydroxypropyl) methacrylamide copolymers Die Makromolekulare Chemie. ,vol. 9, pp. 13- 24 ,(1985) , 10.1002/MACP.1985.020091985103