Synthesis of PLGA nanoparticles of tea polyphenols and their strong in vivo protective effect against chemically induced DNA damage.
作者： Yogeshwer Shukla , Srivastava , Bhatnagar , Singh , Mishra
DOI: 10.2147/IJN.S26364
关键词: DNA 、 In vivo 、 XRCC1 、 DNA repair 、 Pharmacology 、 DMBA 、 Bioavailability 、 Materials science 、 DNA damage 、 Biochemistry 、 Theaflavin
摘要: In spite of proficient results several phytochemicals in preclinical settings, the conversion rate from bench to bedside is not very encouraging. Many reasons are attributed this limited success, including inefficient systemic delivery and bioavailability under vivo conditions. To achieve improved efficacy, polyphenolic constituents black (theaflavin [TF]) green (epigallocatechin-3-gallate [EGCG]) tea poly(lactide-co-glycolide) nanoparticles (PLGA-NPs) were entrapped with entrapment efficacy ~18% 26%, respectively. Further, their preventive potential against 7,12-dimethylbenzanthracene (DMBA)-induced DNA damage mouse skin using alkaline unwinding assay was evaluated. Pretreatment (topically) either TF or EGCG (100 μg/mouse) doses exhibits protection 45.34% 28.32%, respectively, DMBA-induced damage. However, pretreatment TF-loaded PLGA-NPs protects 64.41% by 1/20th dose bulk, 71.79% 1/10th 72.46% 1/5th bulk. Similarly, 51.28% (1/20th bulk), 57.63% (1/10th 63.14% (1/5th bulk) prevention noted EGCG-loaded PLGA-NP doses. These showed that polyphenol-loaded have ~30-fold dose-advantage than bulk Additionally, TF- significant for induction repair genes (XRCC1, XRCC3, ERCC3) suppression responsive (p53, p21, MDM2, GADD45α, COX-2) as compared respective Taken together, a superior ability prevent at much lower concentrations, thus opening new dimension chemoprevention research.
pubpeer.com参考文章(44)
M.C. Hollander, I Alamo, J Jackman, M.G. Wang, O.W. McBride, A.J. Fornace, Analysis of the mammalian gadd45 gene and its response to DNA damage Journal of Biological Chemistry. ,vol. 268, pp. 24385- 24393 ,(1993) , 10.1016/S0021-9258(20)80537-7
Alan D. Lopez, Christopher J. L. Murray, The global burden of disease: a comprehensive assessment of mortality and disability from diseases injuries and risk factors in 1990 and projected to 2020. Harvard School of Public Health on behalf of the World Health Organization and the World Bank , Distributed by Harvard University Press. ,(1996)
Bert Vogelstein, David Lane, Arnold J. Levine, Surfing the p53 network Nature. ,vol. 408, pp. 307- 310 ,(2000) , 10.1038/35042675
Sina Sareth, Shinichi Kitada, Maurizio Pellecchia, Dayong Zhai, John C Reed, Marilisa Leone, Cancer Prevention by Tea Polyphenols Is Linked to Their Direct Inhibition of Antiapoptotic Bcl-2-Family Proteins Cancer Research. ,vol. 63, pp. 8118- 8121 ,(2003)
Donald E. Henson, Sudhir Srivastava, Mukesh Verma, Biomarkers for Early Detection of Colon Cancer Clinical Cancer Research. ,vol. 7, pp. 1118- 1126 ,(2001)
Barbara J. Lyle, Julie A. Mares-Perlman, Barbara E. K. Klein, Ronald Klein, Janet L. Greger, Supplement users differ from nonusers in demographic, lifestyle, dietary and health characteristics. Journal of Nutrition. ,vol. 128, pp. 2355- 2362 ,(1998) , 10.1093/JN/128.12.2355
Sanjay Gupta, Hasan Mukhtar, Chemoprevention of skin cancer through natural agents. Skin Pharmacology and Applied Skin Physiology. ,vol. 14, pp. 373- 385 ,(2001) , 10.1159/000056371
Irena Hreljac, Irena Zajc, Tamara Lah, Metka Filipič, Effects of model organophosphorous pesticides on DNA damage and proliferation of HepG2 cells. Environmental and Molecular Mutagenesis. ,vol. 49, pp. 360- 367 ,(2008) , 10.1002/EM.20392
Preeti Roy, Jasmine George, Smita Srivastava, Shilpa Tyagi, Yogeshwer Shukla, Inhibitory effects of tea polyphenols by targeting cyclooxygenase-2 through regulation of nuclear factor kappa B, Akt and p53 in rat mammary tumors Investigational New Drugs. ,vol. 29, pp. 225- 231 ,(2011) , 10.1007/S10637-009-9349-Y
Nobuhiro Nishiyama, Nanomedicine: nanocarriers shape up for long life. Nature Nanotechnology. ,vol. 2, pp. 203- 204 ,(2007) , 10.1038/NNANO.2007.88