Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification.

摘要: Lentiviruses are associated not only with immunodeficiency but also malignancies. The mechanisms involved in tumorigenesis still fully understood. Cats infected feline virus (FIV) the wild represent one model which role of viral load pathogenesis can be studied, since tumors, especially lymphomas, quite often observed cats FIV. To able to compare data among different FIV isolates, method used obtain has unaffected by isolate-specific differences. This is true for real-time polymerase chain reaction (PCR), a new determination, nucleotide sequence mismatches have been allelic discrimination this method. investigate influence these on PCR efficiency, we an FIV-specific and determined variation several characterized isolates as well unknown from naturally cats. We could demonstrate that minor mismatches, such point mutations primer or probe region, decrease overall efficiency do abolish quantification, contrast major three four nucleotides, lead complete inhibition detection. Based results, it will possible design systems allowing quantification broad range prerequisite investigation impact tumorigenesis.