Sequential injection analysis with chemiluminescence detection for rapid monitoring of commercial Calendula officinalis extractions.

摘要: Introduction Plant extracts containing high levels of antioxidants are desirable due to their reported health benefits. Most techniques capable determining the antioxidant activity plant unsuitable for rapid at-line analysis as they require extensive sample preparation and/or long times. Therefore, analytical real-time or pseudo monitoring extractions, and determination extraction endpoints, would be useful manufacturers antioxidant-rich extracts. Objectives To develop a reliable method in extracts. Materials Methods Calendula officinalis were prepared from dried flowers analysed using sequential injection (SIA) with chemiluminescence (CL) detection. The intensity CL emission reaction acidic potassium permanganate within extract was used signal. SIA-CL applied monitor C. over course batch determine endpoint. Results compared those ultra performance liquid chromatography (UHPLC). Results Pseudo real-time, showed level Calendula plateaued after 100 min extraction. These results correlated well an offline UHPLC study. Conclusion SIA-CL found suitable extractions endpoints respect concentrations. manufacturing industry. Copyright © 2015 John Wiley & Sons, Ltd.