Differential Expression of Genes Involved in Skin Homing, Proliferation, and Apoptosis in CD4+ T Cells of Patients with Atopic Dermatitis
作者： DirkJan Hijnen , Evert Nijhuis , Marjolein de Bruin-Weller , Frank Holstege , Marian Groot Koerkamp
DOI: 10.1111/J.0022-202X.2005.23932.X
关键词: Gene 、 Programmed cell death 、 Immunology 、 Real-time polymerase chain reaction 、 CCR4 、 T lymphocyte 、 Gene expression 、 Microarray 、 Microarray analysis techniques 、 Biology
摘要: CD4+ T cells play a critical role in allergic diseases, both the affected tissue as well systemically. Our objective was to investigate vivo activation state of peripheral blood atopic dermatitis (AD) patients by analyzing gene expression profiles unstimulated cells. mRNA samples from cells, isolated five AD and seven healthy controls (HC), were analyzed using oligonucleotide arrays. Differentially regulated genes validated quantitative PCR (Q-PCR) larger group with AD, asthma (AA), HC subjects. In addition, "typical" helper type 1 (Th1)- Th2-related Q-PCR. Microarray analysis revealed differential 52 patients. Q-PCR confirmed several differentially including CCR10, CRTH2, C-JUN, NR4A2. Two groups highly correlating levels involved homing proliferation or apoptosis, respectively, identified. No marked differences found typical Th1 Th2 AA This study demonstrates that blood, show expressed homing, proliferation, apoptosis. atopy found.
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nature.com参考文章(47)
Steve Rozen, Helen Skaletsky, Primer3 on the WWW for general users and for biologist programmers. Methods of Molecular Biology. ,vol. 132, pp. 365- 386 ,(2000) , 10.1385/1-59259-192-2:365
William O.C.M. Cookson, Baljinder Ubhi, Robert Lawrence, Gonçalo R. Abecasis, Andrew J. Walley, Helen E. Cox, Rosemary Coleman, Nicholas I. Leaves, Richard C. Trembath, Miriam F. Moffatt, John I. Harper, Genetic linkage of childhood atopic dermatitis to psoriasis susceptibility loci Nature Genetics. ,vol. 27, pp. 372- 373 ,(2001) , 10.1038/86867
L. J. Picker, E. C. Butcher, L. S. Rott, S. A. Michie, A unique phenotype of skin-associated lymphocytes in humans. Preferential expression of the HECA-452 epitope by benign and malignant T cells at cutaneous sites. American Journal of Pathology. ,vol. 136, pp. 1053- 1068 ,(1990)
R Disch, K Blaser, C A Akdis, M Akdis, L Weigl, Skin-homing, CLA+ memory T cells are activated in atopic dermatitis and regulate IgE by an IL-13-dominated cytokine pattern: IgG4 counter-regulation by CLA- memory T cells Journal of Immunology. ,vol. 159, pp. 4611- 4619 ,(1997)
M J Birrer, C M Zacharchuk, S A Memon, J D Ashwell, D Petrak, Dominant negative mutant of c-Jun inhibits NF-AT transcriptional activity and prevents IL-2 gene transcription. Journal of Immunology. ,vol. 153, pp. 2046- 2051 ,(1994)
Alice N. McEvoy, Eithne A. Murphy, Tiia Ponnio, Orla M. Conneely, Barry Bresnihan, Oliver FitzGerald, Evelyn P. Murphy, Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-κB and Cyclic Adenosine 5′-Monophosphate Response Element-Binding Protein in Rheumatoid Arthritis Synovial Tissue The Journal of Immunology. ,vol. 168, pp. 2979- 2987 ,(2002) , 10.4049/JIMMUNOL.168.6.2979
Jyothi Rengarajan, Susanne J Szabo, Laurie H Glimcher, Transcriptional regulation of Th1/Th2 polarization. Immunology Today. ,vol. 21, pp. 479- 483 ,(2000) , 10.1016/S0167-5699(00)01712-6
DirkJan Hijnen, Marjolein de Bruin-Weller, Bert Oosting, Cristina Lebre, Esther de Jong, Carla Bruijnzeel-Koomen, Edward Knol, Serum thymus and activation-regulated chemokine (TARC) and cutaneous T cell–attracting chemokine (CTACK) levels in allergic diseases: TARC and CTACK are disease-specific markers for atopic dermatitis The Journal of Allergy and Clinical Immunology. ,vol. 113, pp. 334- 340 ,(2004) , 10.1016/J.JACI.2003.12.007
AXEL TRAUTMANN, SVEN KLUNKER, ISABELLE DAIGLE, UMUT C. KÜÇÜUKSEZER, WOLFGANG DEGLMANN, RAINER DISCH, KURT BLASER, CEZMI A. AKDIS, MÜBECCEL AKDIS, T helper (Th) 2 predominance in atopic diseases is due to preferential apoptosis of circulating memory/effector Th1 cells The FASEB Journal. ,vol. 17, pp. 1026- 1035 ,(2003) , 10.1096/FJ.02-1070COM
Markus Grewe, Carla A.F.M Bruijnzeel-Koomen, Erwin Schöpf, Theo Thepen, Alice G Langeveld-Wildschut, Thomas Ruzicka, Jean Krutmann, A Role for Th1 and Th2 Cells in the Immunopathogenesis of Atopic Dermatitis Immunology Today. ,vol. 19, pp. 359- 361 ,(1998) , 10.1016/S0167-5699(98)01285-7