Studies on the bacteriophage MS2. Some nucleotide sequences from the RNA-polymerase gene

摘要: The knowledge of the amino acid sequence from coat protein RNA bacteriophages f2, R 17 and MS2 [ l-31 allowed identification several fragments as being derived this gene. They were all obtained by partial ribonuclease hydrolysis R17 [4-61, f2 [7] or [8, 91. For MS2, these sequences could be further extended, complete gene was established [lo]. Furthermore, se- quence followed a 36 nucleotides long inter- cistronic region ribosomal binding site next gene, RNA-polymerase. As for two other prod- ucts, A-protein viral RNA-polymerase, are not known, no such simple test exists to identify polynucleotides corresponding latter genes. In communication we present data on three nucleotide polymerase This conclusion is based direct overlaps with known lo] , order phages 11, 121, mapping series polypurine tracts in molecule 131 . 2. Methods All described study *