A Distinct Nuclear Import Pathway Used by Ribosomal Proteins
作者： Michael P Rout , Günter Blobel , John D Aitchison
DOI: 10.1016/S0092-8674(00)80254-8
关键词: Nucleoporin 、 Cell biology 、 Nuclear transport 、 Nuclear export signal 、 Biology 、 Karyopherin 、 Nuclear protein 、 Nuclear pore 、 Molecular biology 、 Importin 、 Ribosomal protein 、 General Biochemistry, Genetics and Molecular Biology
摘要: Protein transport into the nucleus is governed by interaction of soluble factors with their import substrates and nuclear pore complexes. Here, we identify a major distinct pathway, mediated previously uncharacterized yeast beta karyopherin Kap123p. The predominant for this pathway are ribosomal proteins, which must be imported prior to assembly pre-ribosomes. Kap123p binds directly its substrates, repeat motif-containing nucleoporins, Ran-GTP. We show that related protein Pse1p also can functionally substitute Kap123p; both capable specifically directing localization signal reporter in vivo.
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