Eupolybothrus cavernicolus Komerički & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae): the first eukaryotic species description combining transcriptomic, DNA barcoding and micro-CT imaging data.

摘要: We demonstrate how a classical taxonomic description of new species can be enhanced by applying generation molecular methods, and novel computing imaging technologies. A cave-dwelling centipede, Eupolybothrus cavernicolus Komericki & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae), found in remote karst region Knin, Croatia, is the first eukaryotic for which, addition to traditional morphological description, we provide fully sequenced transcriptome, DNA barcode, detailed anatomical X-ray microtomography (micro-CT) scans, movie living specimen document important traits its ex-situ behaviour. By employing micro-CT scanning time, create high-resolution dataset that allows virtual reconstructions subsequent interactive manipulation test recently introduced ‘cybertype’ notion. In addition, transcriptome was recorded with total 67,785 scaffolds, having an average length 812 bp N50 1,448 (see GigaDB). Subsequent annotation 22,866 scaffolds conducted tracing homologs against current available databases, including Nr, SwissProt COG. This pilot project illustrates workflow producing, storing, publishing disseminating large data sets associated taxon. All have been deposited publicly accessible repositories, such as GigaScience GigaDB, NCBI, BOLD, Morphbank Morphosource, respective open licenses used ensure their accessibility re-usability.