Mechanisms of the Antiinflammatory Effects of α‐MSH: Role of Transcription Factor NF‐κB and Adhesion Molecule Expression

摘要: The recruitment of leukocytes from the circulation to inflamed tissue is regulated by expression adhesion molecules on both and endothelial cells. proopiomelanocortin-derived peptide alpha-melanocyte stimulating hormone (alpha-MSH) known modulate inflammation. Thus, we investigated influence alpha-MSH LPS-induced E-selectin VCAM-1 Human microvascular cells (HMEC-1) were treated with LPS (100 ng/ml) alone or in presence (10(-8) 10(-16) M). RT-PCR analysis showed that significantly reduced E-selectin. Since many contain regulatory NF-kappa B sites their promoter region, role activation transcription factor NF-alpha was also investigated. downregulated LPS-mediated B, a dose-dependent manner. These findings indicate modulation crucial molecular event, one seems be responsible for antiinflammatory effects alpha-MSH.