p53-dependent apoptosis in the absence of transcriptional activation of p53-target genes.

作者: Carme Caelles , Arno Helmberg , Michael Karin

DOI: 10.1038/370220A0

关键词: Programmed cell deathCell biologyApoptosisRegulation of gene expressionImmunologyTumor suppressor geneGeneTranscription (biology)Activator (genetics)BiologyDNA damage

摘要: The tumour suppressor p53 is required to induce programmed cell death (apoptosis) by DNA-damaging agents. As a transcriptional activator that mediates gene induction after DNA damage, it has been proposed be genetic switch activates apoptosis-mediator genes. Here we evaluate the role of in DNA-damage-induced apoptosis establishing derivatives GHFT1 cells, are somatotropic progenitors immortalized expression SV40 T-antigen, which express temperature-sensitive mutant. In these cells damage depends strictly on function. A shift permissive temperature triggers following but this independent new RNA or protein synthesis. extent apoptotic cleavage directly proportional period during functional. These results do not support proposal an genes rather indicate either represses necessary for survival component enzymatic machinery repair DNA.

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