Effect of Initial Glucose Concentrations on Carbon and Energy Balances in Hydrogen-Producing Clostridium tyrobutyricum JM1

摘要: 333.6 mM). Because an understandingof metabolic regulations was required to provide guidancefor further effective design or optimization, in thiscase, maximizing hydrogen production, carbon and energybalances by C. tyrobutyricum JM1 were determined andapplied anaerobic glucose metabolism. The overall carbondistribution suggested that initial concentrationshad strong influence on the stoichiometric coefficientsof products molar production of ATP theformation biomass. had a highcapacity for at glucoseconcentration 222.4 mM with high concentrations ofacetate butyrate.Keywords: Clostridium tyrobutyricum, material balance,energy balance, flow distribution, productionSaccharolytic clostridia represent one largest generaof prokaryotes satisfy following four criteria: (a)able form endospores; (b) must rely energymetabolism obligate anaerobes; (c) unable carry out adissimilatory sulfate reduction; (d) cell wall mustbe Gram-positive [2]. ofthe saccharolytic clostridia, is low-G+C Gram-positiveanaerobe exhibits special routes produceshort-chain fatty acids gas from carbohydratesand amino [2, 13]. Hydrogen recognized as arenewable promising energy alternative futurebecause it does not emit carbon-based gases hashigh specific content per unit mass [12]. Amongbiological processes, fermentativehydrogen proceeds anaerobicmetabolism pyruvate electron transferchains: pyruvate:ferredoxin oxidoreductase (PFOR), NADH:ferredoxin (NFOR), hydrogenase [23].PFOR oxidizes acetyl-CoA CO