Improved Butanol–Methanol (BUME) Method by Replacing Acetic Acid for Lipid Extraction of Biological Samples
作者： Mutya Cruz , Miao Wang , Jessica Frisch-Daiello , Xianlin Han
DOI: 10.1007/S11745-016-4164-7
关键词: Lipidomics 、 Mass spectrometry 、 Extraction (chemistry) 、 Lithium chloride 、 Shotgun lipidomics 、 Acetic acid 、 Chemistry 、 Methanol 、 Butanol 、 Chromatography
摘要: Extraction of lipids from biological samples is a critical step in lipidomics, especially for shotgun lipidomics where lipid extracts are directly infused into mass spectrometer. The butanol–methanol (BUME) extraction method was originally developed to extract plasma with 1 % acetic acid. Considering some sensitive acidic environments, we modified this protocol by replacing acid lithium chloride solution and extended the tissue samples. Although no significant reduction plasmalogen levels BUME rat heart found, established various samples, including liver, heart, plasma. Essentially identical profiles majority classes were obtained traditional Bligh–Dyer methods. However, it found that neither original, nor suitable 4-hydroxyalkenal species measurement
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