KINETICS OF AVAILABLE LYSINE LOSSES IN A SOY PROTEIN ISOLATE: CONFIRMATION OF THE ‘TRANSITION PHASE’ BY PROTEIN EFFICIENCY RATIO TESTS

作者: J. c. WOLF , D. R. THOMPSON , P. C. Ahn , P. V. J. HEGARTY

DOI: 10.1111/J.1365-2621.1979.TB10067.X

关键词: SugarLysineProtein efficiency ratioPhase (matter)Transition (genetics)KineticsChromatographySoy proteinChemistry

摘要: Available lysine losses in soy protein isolates during thermal processing go through three phases: a fist-order loss, transition phase and no-loss phase. The shows statistically significant (P < 0.02) increase available when measured by the fluoro-dinitrobenzene (FDNB) method. Using efficiency ratio method (PER), 0.1) was demonstrated samples processed at 80° C for 700 mm. PER values decreased from 2.65 ± 0.04 to 1.17 after 600 min of processing. At value increased 1.37 0.05. 80°C systems containing 4% sugar occurs mm is initiated according previously published equations. FDNB results exhibited an excellent correlation (r = 0.98). Thus, confirm detected using procedure.

参考文章(8)
Philip M-B. Leung, Quinton R. Rogers, Alfred E. Harper, Effect of Amino Acid Imbalance on Dietary Choice in the Rat Journal of Nutrition. ,vol. 95, pp. 483- 492 ,(1968) , 10.1093/JN/95.3.483
K. J. Carpenter, The estimation of the available lysine in animal-protein foods Biochemical Journal. ,vol. 77, pp. 604- 610 ,(1960) , 10.1042/BJ0770604
V. H. Booth, Problems in the determination of FDNB-available lysine. Journal of the Science of Food and Agriculture. ,vol. 22, pp. 658- 666 ,(1971) , 10.1002/JSFA.2740221214
Amal M. Boctor, A. E. Harper, Measurement of available lysine in heated and unheated foodstuffs by chemical and biological methods. Journal of Nutrition. ,vol. 94, pp. 289- 296 ,(1968) , 10.1093/JN/94.3.289
C.H. Lea, R.S. Hannan, Studies of the reaction between proteins and reducing sugars in the “dry” state Biochimica et Biophysica Acta. ,vol. 3, pp. 313- 325 ,(1949) , 10.1016/0006-3002(49)90100-6